Construction and screening of mutant strains

更新时间：2025/6/26 上传企业：上海金鹏分析仪器有限公司点击：27 下载：0次

关键词：Shake-flask fermentation and cell growth

概述：Cell growths were monitored every 8 h for reading at 600 nm using the nana-800 + ultra microspectrophotometer (Shanghai JP Analytical Instrument Co., Ltd., Shanghai, China).

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PCR products were subjected to agarose gel electrophoresis and the separated DNA fragments were intercalated with ethidium bromide for visualization using the Gel Imaging Analysis Systems JP-2880 (Shanghai JP Analytical Instrument Co., Ltd., Shanghai, China).