Cell growths were monitored every 8 h for reading at 600 nm using the nana-800 + ultra microspectrophotometer (Shanghai JP Analytical Instrument Co., Ltd., Shanghai, China).
The harvested cells were resuspended in the lysis buffer con taining 50 mM Tris–HCl (pH 8.0), 100 mM NaCl, 1 mM CuCl2, 1 mg/L of lysozyme, and 10 µg/L of benzonase.