labeling Antibodies with (Ln3+)n-DNA Tags

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a Nano-800 (Shanghai JP Analytical Instrument Co., Ltd.) spectrophotometer and stored at −80 °C.

we also used the Nano-800 spectrophotometer to evaluate the products at each stage. The absorbance ratio between 260 and 280 nm (A260/A280) serves as a critical metric for assessing the purity of DNA or antibodies. Following the conjugation with SCN-DTPA and the DBCO-Mal linker, the A260/A280 ratio of (Ln3+)n-DNA exhibited significant shifts (Figure 1B), confirming effective conjugation of the components to (amino)n-DNA. Finally,