a protein purifica-tion system

更新时间：2025/6/24 上传企业：上海金鹏分析仪器有限公司点击：161 下载：0次

关键词：分离纯化

概述：The harvested cells were resuspended in the lysis buffer con taining 50 mM Tris–HCl (pH 8.0), 100 mM NaCl, 1 mM CuCl2, 1 mg/L of lysozyme, and 10 µg/L of benzonase.

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The harvested cells were resuspended in the lysis buffer containing 50 mM Tris–HCl (pH 8.0), 100 mM NaCl, 1 mM CuCl2, 1 mg/L of lysozyme, and 10 µg/L of benzonase. After 30-min incubation at ambient temperature, the cells were sonicated and the lysates were clarified by centrifugation at 8000 g for 20 min at 4 °C, and then loaded onto an immobilized metal affinity chromatography (IMAC) column (BioRad, Hercules, CA, USA) equipped with a protein purification system (JP-blupadStar, Shanghai, China).